By Keith B. Glaser, Mark D. Lister, Richard J. Ulevitch, Edward A. Dennis (auth.), Patrick Y-K Wong, Edward A. Dennis (eds.)
ISBN-10: 1468458051
ISBN-13: 9781468458053
ISBN-10: 1468458078
ISBN-13: 9781468458077
This quantity within the Advances in Experimental medication and Biology sequence is devoted to constructing an total view of the "state-of the-art" of data within the box of phospholipase A2 and to exploring the function and serve as of this enzyme in a number of inflammatory ailments. This e-book grew out of 2 significant symposia on phospholipase A2 held in 1989: I). "Phospholipase A2: Pathophysiological position of Soluble and Membrane-Bound Enzymes" equipped via Dr. Doug Morgan and Dr. Ann Welton of Hoffmann-La Roche and subsidized by means of the hot York Academy of Sciences (January 24, 1989). II). "Release and serve as of Phospholipase A2 from Inflammatory Cells" equipped via Dr. Patrick Wong and Dr. Edward Dennis on the FASEB assembly in New Orleans (March 22, 1989). Readers will locate intriguing advances in our knowing of the constitution, functionality and molecular biology of phospholipase A2 study that's provided during this quantity. The elucidation of gene buildings of phospholipase A2 should still result in new insights and new approches within the keep an eye on and law of the enzyme job. this can be basic to higher figuring out the functionality of phospholipase A2 and the choice of its task in mobile and animal structures. of serious value is the advance of animal types for inflammatory sickness which will evaluation the function of phospholipase A2 and its inhibitors. we're confident that this quantity might be an outstanding reference that surveys this box during which a variety of investigators are presently actively at work.
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Frank, P. Vadas, V. J. K. Johnson, Chromosomal mapping of human synovial fluid PLA2 and a related exon, manuscript in preparation. A. B. H. D. Brow, DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA, Biochemistry 85:9436 (1988). S. Mount, A catalogue of splice junction sequences. Nucleic Acids Res. 10:459 (1982). J. Maranganore and R. Heindrikson, The Lysine-49 phospholipase A2 from the venom of Agkistrodon piscivorus piscivorus: relation of structure and function to other phospholipases A2, J.
A, Kloss, J. Chem. M. H. , eds) Vol 4, pp. , Amsterdam Snyder, F. Rev. X. & Klein, M. S. (1978) J. Clin. Invest. J. L. Chem. R & Authi, KS. Acta 665, 571-577 Vadas, P. & Pruzanski, W. lnvest. , Farewell, V. & Bombardier, C. Care Med. , Pruzanski, W. & Stefanski, E. , Kim, J. & Fornasier, V. Pathol. 134,807-811 Van den Bosch, H. M. & Pieroni, G. , Slotboom, AJ. H. Physiol. Pharmacol. 91,91-203 Waite, M. , Miura, K. & Nozawa, Y. J. M. M. Dis. 136, 204-207 53 PURIFICATION AND CHARACTERIZATION OF A PHOSPHOLIPASE A2 FROM HUMAN OSTEOARTHRITIC SYNOVIAL FLUID Thomas P.
E Q. : o B 80 ,: Fraction Number Fig. 2. coli substrate) from S-Sepharose (A), Sephadex G-50 (B) and C4-reverse-phase HPLC (C) columns. A 1 ~' r----------------, CoCI: (m"') Fig. 3. B 15,, - - - - - - - - - - - - . PLA2 activity. R. M. KRAMER ET AL. 40 human genomic phage library by plaque hybridization analysis. One clone hybridized to both probes and contained a genomic DNA insert of 15 kb. 2-kb HindIII restriction fragment that hybridized to both probes. The restriction fragment was subcloned and completely sequenced from both strands.
Phospholipase A2 : Role and Function in Inflammation by Keith B. Glaser, Mark D. Lister, Richard J. Ulevitch, Edward A. Dennis (auth.), Patrick Y-K Wong, Edward A. Dennis (eds.)
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