Bruce D. Jensen (auth.), Alain Jacquemin-Sablon (eds.)'s Flow Cytometry: New Developments PDF

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G. B. F. Boron. " Nature 337 (1989): 648-651. , M. Poenie, and R. Y. Tsien. " Journal of Biological Chemistry (2601985): 3440-3450. P. (1991) Bioprobes. Volume 13, April. Molecular Probes, Inc. Eugene, Oregon, USA. B. (1980) Localization of mtiochondria in living cells with rhodamine 123. Proceedings of the National Academy of Sciences (USA) 77:990998. w. (1988) Activation of Ca++ dependent K+ channels in human B lymphocytes by anti-immunoglobulin. Journal of Clinical Investigation 81: 449-454.

2-D frequency contour plot of fluorescence versus time. B. Sequential fluorescence histograms for the 2nd, 4th and 8th of 8 equal time slices, the overall time being 5 minutes. c. 3-D display of fluorescence versus time versus forward scatter (size). 0~M, curves we for took substrate the median 36 fluorescence of each of 8 equal time slices throughout the 5 minute reaction. Plotting the reciprocal of the initial velocity versus the reciprocal of substrate concentration (the Lineweaver-Burke Plot) Michaelis constant for enables calculation the reaction of (Figure the 4, apparent Dive et al 1987a) .

0 S ::1 Z FLl Figure 5 : Flow cytometry analysis of the internalization of fluoresceinylated and mannosylated bovine serum albumin (F-,Man-BSA) by macro phages. (A) cell autofluorescence; (B) cell fluorescence intensity of macrophages incubated at 4°C with F-,Man-BSA; (C) cell fluorescence intensity of macrophages incubated at 37°C with F-,Man-BSA; (0) cell fluorescence intensity of macrophages incubated at 3rC, washed and further incubated at 4°C for 30 minutes in the presence of 50 ",M monensin.

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