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Electron Microscopy of Plant Pathogens by H. C. Hoch (auth.), Prof. Dr.rer.nat. Kurt Mendgen, Dr. PDF

By H. C. Hoch (auth.), Prof. Dr.rer.nat. Kurt Mendgen, Dr. Dietrich-Eckhardt Lesemann (eds.)

ISBN-10: 3642758185

ISBN-13: 9783642758188

ISBN-10: 3642758207

ISBN-13: 9783642758201

Plants, fungi, and viruses have been one of the first organic gadgets studied with an electron microscope. one of many first tools equipped by way of Siemens used to be utilized by Helmut Ruska, a brother of Ernst Ruska, the pioneer in developing electron microscopes. H. Ruska released a variety of papers on diversified organic gadgets in 1939. in a single of those, the photographs via G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a brand new age in microscopy. the most challenge was once then because it nonetheless is this present day, to procure a suitable practise of the specimen for commentary within the electron microscope. Beam harm and specimen thickness have been the 1st hindrances to be met. L. Marton in Brussels not just outfitted his personal software, but additionally made huge development in specimen education by means of introducing the impregnation of samples with heavy metals to acquire worthwhile distinction. His photographs of the fowl nest orchid root impregnated with osmium have been progressive while released in 1934. it isn't where right here to bear in mind the various thoughts which have been constructed within the next years to realize the fashionable wisdom at the fantastic constitution of plant cells and of alternative plant pathogens. The great development got with tobacco mosaic virus is mirrored within the bankruptcy through M. Wurtz at the advantageous constitution of viruses during this quantity. New cytochemical and immunological thoughts significantly surpass the morphological details acquired from the pathogens, in particular on the host-parasite interface.

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Additional resources for Electron Microscopy of Plant Pathogens

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The extrahaustorial membrane around haustoria of U. viciae fabae is surrounded by small "coated" tubules; the haustorial wall (fw) has uneven staining properties (Fig. 5d). A molecular characterization of the haustorial host-parasite interface was obtained with lectins. Sections from tissue without chemical fixation embedded in Lowicryl K 11 M were incubated with the gold-labeled lectin Concanavalin A (Con A-Gold) (Fig. 5e) and that of Arachis hypogea (PNA-Gold) (Fig. 5f) as described by Rohringer et al.

After infiltration of bean leaves with water (Table 1, Exp. 3) or wheat leaves with buffer (Table 1, Exp. 17) serious damage, probably as a result of ice crystal formation, was ovious throughout the leaf parenchyma. Less than 10% of the mesophyll cells remained intact. In these few cells, haustoria exhibited a wellpreserved fine structure. In the bundles, many phloem parenchyma cells remained intact (Fig. 4a). The cytoplasm in some intercellular hyphae was well preserved. Good results were obtained with all the hyphae of the sporogenous tissue.

7, 9, 11, 12). , organelles, macromolecular structures, solutes and some hydration water) [6,25,26]. The extent of ice crystal damage determines the spatial resolution achievable within fractured cells using LTSEM. Nevertheless, useful structural information is often evident at the subcellular level. One useful feature of this artifact is that it can give one a qualitative idea of amounts offreezeable water in different parts of a cell; the more widely spaced the segregation zones, the higher the freeze able water content [6].

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Electron Microscopy of Plant Pathogens by H. C. Hoch (auth.), Prof. Dr.rer.nat. Kurt Mendgen, Dr. Dietrich-Eckhardt Lesemann (eds.)


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