By James F. Rusling
ISBN-10: 0824708997
ISBN-13: 9780824708993
Examines movies of biomolecules which can supply sturdy surfaces for catalyzing enzyme reactions, serve in biosensors and as biorecognition components, mediate nanoparticle formation, and supply a simple for primary reviews and functions in biomedical units.
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Extra resources for Biomolecular Films Design Function and Applications
Example text
1 M solution of a soluble ionic surfactant. The adsorbed layer of ordered surfactant or lipid inhibits macromolecule adsorption, and maintains a viable path for electron exchange between the electrode and the protein. The type of surfactant greatly in£uences E00 of the protein in these ¢lms. Values in DDAB ¢lms are the most positive and closest to reported solution E00 s for Mb [7], although still about 50 mV negative of solution values. Values in DDAB ¢lms are about Copyright 2003 by Marcel Dekker, Inc.
2 DNAÀIonomer Films Films of double-stranded (ds) DNA and ionomers were stable enough to be used for detecting DNA damage from incubation of the ¢lm with styrene oxide, which reacts with guanine bases and disrupts the double helix. Films were prepared on PG electrodes by casting mixtures of ds-DNA with Eastman AQ38S or with Na¢on [13,121]. Damage to these DNA ¢lms was detected by oxidation peaks that developed during reaction with styrene oxide at 37 C for 10 min to several hours using derivative square-wave voltammetry.
Current is sampled at the end of the each forward and backward pulse, and plotted as forward, reverse, and di¡erence current versus step potential. Di¡erence square-wave voltammograms typically lead to symmetric peaks resulting from real-time subtraction of currents measured at the end of each forward and reverse pulse. An example of a reversible square-wave voltammogram is shown for a thin ¢lm of the electroactive polymer bis-bipyridylruthenium(II)-poly (vinylpyridine) [Ru(bpy)2 -PVP]2þ (Fig. 5), depicting the forward, reverse, and di¡erence current curves [58].
Biomolecular Films Design Function and Applications by James F. Rusling
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